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Question: Emilía and Aron, siblings and both of Icelandic heritage, decided to send their DNA samples for t…


Emilía and Aron, siblings and both of Icelandic heritage, decided to send their DNA samples for testing to determine whether they had any DNA mutations that might be linked to diseases Sanger sequencing was performed and the sequencing gel for the 225th to 245th nucleotide positions of the coding sequence of the MYL4 gene is shown below for a normal individual, for Emilía, and for Aron. [The nucleotides at bottom indicate the ddNTP used in the reaction] Normal Emilia Aron 245 드 225 G CA T G C A T G CA T c- 1pt) Using the data from the Normal sequencing gel deduce the template sequence for the sequencing reaction (indicate the 3 and 5 ends of the template):

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Emilía and Aron, siblings and both of Icelandic heritage, decided to send their DNA samples for testing to determine whether they had any DNA mutations that might be linked to diseases Sanger sequencing was performed and the sequencing gel for the 225th to 245th nucleotide positions of the coding sequence of the MYL4 gene is shown below for a normal individual, for Emilía, and for Aron. [The nucleotides at bottom indicate the ddNTP used in the reaction] Normal Emilia Aron 245 드 225 G CA T G C A T G CA T c- 1pt) Using the data from the 'Normal' sequencing gel deduce the 'template' sequence for the sequencing reaction (indicate the 3' and 5' ends of the template):

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