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Question: I had a question about restriction sites and fragments of lambda
dna cut with hindiii


I had a question about restriction sites and fragments of lambda
dna cut with hindiiiTable 11-1: Restriction sites and fragments of lambda DNA cut with HindIll HindIII Restriction 7 End sites Position of23130 25157 27479 36895 37459 3758444141 cut in bp Size of DNA 23130 to left of cut 2027 Order by size 2 - Paper exercise from professional 1 - Determination of DNA fragment ones arent there. They are too small to for this gel sizes from relative movement on gel 2. Measuring distanc DNA fragments move through a gel more rapidly the smaller they are, but how far depends on a number of variables. They move DNA in cach band of the HindilI digest moved faster if the current is stronger or the agarose from the front of the well. Record this in Table thinner. The DNA moves further the longer the 11-2. This and the DNA size in the bands is gel is run. However, on a single gel all of these your known. These are the two variables you variables should be the same, so if there are will use to generate a standard curve. fragments of known sizes being run in one lane Next measure how far the DNA fragments I digests. Enter vement can be compared to unknown DNA bands in other lanes. In this exercise, we moved in the EcoRI and BamH know the lengths of the fragmentofrom the these into Table 11-2. You dont know the HindIII digest of lambda which you calculated fragment size in Table 11-1. Therefore, if you use it as your standard, you can calculate the size in base 3. Drawing a standard curve páirs of the fragments in the bands generated when lambda DNA was digested by EcoRI and Draw a graph comparing how far the DNA BamHI. Use the photograph in Figure 11-2 to estimate the length of the restriction fragments to their size. This should indicate for these generated by EcoRI and BamHI digests. fragments in the HindlI digest moved relative conditions how far a fragment will move. Use the semi-log paper provided because there are I. Determining fragment size in known band In Table 11-2 enter all of the fragment sizes produced when lambda DNA is digested with Hindlll (from Table 11-I). When you try locating eight fragments on the gel you will find only six. This is because the two smallest such big differences in the fragment sizes. Use the distances as the independent variable. After drawing the graph, determine whether there appears to be a correlation. Connect the dots. The line produced, a best-fit line, should indicate the relationship between size and movement for any distance on the gel

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Table 11-1: Restriction sites and fragments of lambda DNA cut with HindIll HindIII Restriction 7 End sites Position of23130 25157 27479 36895 37459 3758444141 cut in bp Size of DNA 23130 to left of cut 2027 Order by size 2 – Paper exercise from professional 1 – Determination of DNA fragment ones aren't there. They are too small to for this gel sizes from relative movement on gel 2. Measuring distanc DNA fragments move through a gel more rapidly the smaller they are, but how far depends on a number of variables. They move DNA in cach band of the HindilI digest moved faster if the current is stronger or the agarose from the front of the well. Record this in Table thinner. The DNA moves further the longer the 11-2. This and the DNA size in the bands is gel is run. However, on a single gel all of these your known. These are the two variables you variables should be the same, so if there are will use to generate a standard curve. fragments of known sizes being run in one lane Next measure how far the DNA fragments I digests. Enter vement can be compared to unknown DNA bands in other lanes. In this exercise, we moved in the EcoRI and BamH know the lengths of the fragmentofrom the these into Table 11-2. You don't know the HindIII digest of lambda which you calculated fragment size in Table 11-1. Therefore, if you use it as your standard, you can calculate the size in base 3. Drawing a standard curve páirs of the fragments in the bands generated when lambda DNA was digested by EcoRI and Draw a graph comparing how far the DNA BamHI. Use the photograph in Figure 11-2 to estimate the length of the restriction fragments to their size. This should indicate for these generated by EcoRI and BamHI digests. fragments in the HindlI digest moved relative conditions how far a fragment will move. Use the semi-log paper provided because there are I. Determining fragment size in known band In Table 11-2 enter all of the fragment sizes produced when lambda DNA is digested with Hindlll (from Table 11-I). When you try locating eight fragments on the gel you will find only six. This is because the two smallest such big differences in the fragment sizes. Use the distances as the independent variable. After drawing the graph, determine whether there appears to be a correlation. Connect the dots. The line produced, a best-fit line, should indicate the relationship between size and movement for any distance on the gel

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