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Question: A researcher is conducting experiments to characterize an unknown enzyme. In order to determine t…


A researcher is conducting experiments to characterize an unknown enzyme. In order to determine the total protein content in the sample, she performed a Bradford Assay For her standard curve, she added 20 μL of each peptide solution to the dye. She then used the data in the table below to create her standard curve Bradford Protein Assay Standard Curve Concentration (ug/mL) 82.0 61.5 41.0 32.8 24.6 16.4 8.2 0.0 Absorbance@595 nm 0.880 0.744 0.609 0.401 0.351 0.233 0.121 0.000 BSA Standard Curve (20 μL sample, 3 mL Bradford Reagent) 1.200 1.000 R 0.800 0.600 0.400 0.200 y 0.0119x R2 0.9552 0.000 0.0 10.0 20.0 30.0 40.0 50.0 60.070.0 80.0 90.0 Concentration of BSA (Hg/uL) Screenshot Screen Shot 2018-10-11 at 11.21.44 PM 2a. (5 pts.) Based on the researchers standard curve, what concentration range of nown samples can she measure? unk 2b. (5 pts.) Breifly describe what should be done if the researcher finds that the absorbance of her unknown sample is outside of the linear range of her standard curve 2c. (10 pts.) Determine the concentration (uM) of protein in a enzyme sample if 20 μL was added to 3 mL of Bradford reagent and the absorbance was 0.750 at 595 nm Assume the sample was pure 2d. (5 pts.) Why is protein concentration determined by a Bradford Assay consideredia relative protein concentration? 2e. (5 pts.) What is the most common protein used to make the standa Bradford Assays? Why are the benefits of using this protein? Screenshot Screen Shot 2018-10-11 at 11.22.18 PM

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A researcher is conducting experiments to characterize an unknown enzyme. In order to determine the total protein content in the sample, she performed a Bradford Assay For her standard curve, she added 20 μL of each peptide solution to the dye. She then used the data in the table below to create her standard curve Bradford Protein Assay Standard Curve Concentration (ug/mL) 82.0 61.5 41.0 32.8 24.6 16.4 8.2 0.0 Absorbance@595 nm 0.880 0.744 0.609 0.401 0.351 0.233 0.121 0.000 BSA Standard Curve (20 μL sample, 3 mL Bradford Reagent) 1.200 1.000 R 0.800 0.600 0.400 0.200 y 0.0119x R2 0.9552 0.000 0.0 10.0 20.0 30.0 40.0 50.0 60.070.0 80.0 90.0 Concentration of BSA (Hg/uL) Screenshot Screen Shot 2018-10-11 at 11.21.44 PM 2a. (5 pts.) Based on the researchers standard curve, what concentration range of nown samples can she measure? unk 2b. (5 pts.) Breifly describe what should be done if the researcher finds that the absorbance of her unknown sample is outside of the linear range of her standard curve 2c. (10 pts.) Determine the concentration (uM) of protein in a enzyme sample if 20 μL was added to 3 mL of Bradford reagent and the absorbance was 0.750 at 595 nm Assume the sample was pure 2d. (5 pts.) Why is protein concentration determined by a Bradford Assay consideredia "relative protein concentration"? 2e. (5 pts.) What is the most common protein used to make the standa Bradford Assays? Why are the benefits of using this protein? Screenshot Screen Shot 2018-10-11 at 11.22.18 PM

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