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Question: You successfully purify a protein and start to do various
experiments to characterize it. When yo…


You successfully purify a protein and start to do various
experiments to characterize it. When you get an estimate of it’s
molecular weight using gel filtration (size exclusion)
chromatography you find that it weighs 65 kDa. You next run it
using SDS PAGE and find two bands, one corresponding to 25 kDa and
one band weighing 40 kDa. Can you explain these results?

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