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Question: You are breaking up cells and isolating plasma membrane. During
the process, you are only able to…


You are breaking up cells and isolating plasma membrane. During
the process, you are only able to isolate pieces of plasma membrane
in the form of vesicles. The vesicles are either right-side-out or
inside-out in your preparations. To separate the two types of
vesicles, a friend suggest that you pass your vesicles over an
affinity column made of lectin, a sugar binding molecule. What is
the point?

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