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Question: St of Enzyme Concentration on Activit Materials Test tubses and test tobe ck si’..cticy,botomcici…


st of Enzyme Concentration on Activit Materials Test tubses and test tobe ck si..cticy,botomcici lulx, sor.inc..cs petroplhotomcte Phosphate bufler solution (p17 0) Hydrogen peroxide solation OPD solotion tuzyme extract from potato Gİ.HI..ate pipette ind pipete Pump Micropipette and mietopipette tip Procedure TUBE CRIS l (blank) Coutestacat 410m 0.1 . Number 5 clean test tubes (or spectrophotometer tube), 1 through 5 2. Load all five tubes with the buller, hydrogen peroxide, and OlD in the order listed. The amounts 3. Add the enzyme to cach tube sequentially with I min interval (important the moment you add 4. Vortex the tubes gently when all contents have been added 5. Incubate cach tube at room temperature for 5 minutes to add to cach tube are provided in the table above the enzyme, the enzymatic reaction starts and this is time zero of the reaction) Questions: Why should we have a 1 minute interval ere Questions: Do you observe the color change in your tubes? Do you observe any air bubble in your tubes? Measure the absorbency (A) at 410 nm of cach tube using tube # I as your blank to calibrate the spectrophotometer. (Vortex your tube before determining absorbency) lot the absorbency (Y axis) versus the amount of enzyne added to the tubes (X axis) on the grapth . Record your results in the table above (Absorbency column paper provided at the end of this chapter.J

  

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st of Enzyme Concentration on Activit Materials Test tubses and test tobe ck si'..cticy,botomcici lulx, sor.inc..cs petroplhotomcte Phosphate bufler solution (p17 0) Hydrogen peroxide solation OPD solotion tuzyme extract from potato Gİ.HI..ate pipette ind pipe'te Pump Micropipette and mietopipette tip Procedure TUBE CRIS l (blank) Coutestacat 410m 0.1 . Number 5 clean test tubes (or spectrophotometer tube), 1 through 5 2. Load all five tubes with the buller, hydrogen peroxide, and Ol'D in the order listed. The amounts 3. Add the enzyme to cach tube sequentially with I min interval (important the moment you add 4. Vortex the tubes gently when all contents have been added 5. Incubate cach tube at room temperature for 5 minutes to add to cach tube are provided in the table above the enzyme, the enzymatic reaction starts and this is time zero of the reaction) Questions: Why should we have a 1 minute interval ere Questions: Do you observe the color change in your tubes? Do you observe any air bubble in your tubes? Measure the absorbency (A) at 410 nm of cach tube using tube # I as your blank to calibrate the spectrophotometer. (Vortex your tube before determining absorbency) lot the absorbency (Y axis) versus the amount of enzyne added to the tubes (X axis) on the grapth ". Record your results in the table above (Absorbency column paper provided at the end of this chapter.J

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