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Question: 7. The following double reciprocal plot below describes the effect of an inhibitor on enzyme acti…


7. The following double reciprocal plot below describes the effect of an inhibitor on enzyme activity. With inhibitor 50x 10* 0 0105 y 3.0x30+0.010 Note: units for velocity are umol/min and units for substrate concentration are M Determine: a) apparent Vmax in the presence and the absence of an inhibitor b) apparent Km in the presence and the absence of an inhibitor c) Ki and/orkK d) type of inhibition Describe how the inhibitor interacts and affects the enzyme based on the type of inhibition you determined. 15 points] Page 6 of 6

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7. The following double reciprocal plot below describes the effect of an inhibitor on enzyme activity. With inhibitor 50x 10* 0 0105 y 3.0×30+0.010 Note: units for velocity are umol/min and units for substrate concentration are M Determine: a) apparent Vmax in the presence and the absence of an inhibitor b) apparent Km in the presence and the absence of an inhibitor c) Ki and/orkK d) type of inhibition Describe how the inhibitor interacts and affects the enzyme based on the type of inhibition you determined. 15 points] Page 6 of 6

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