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Question: QUESTION B:
A patch clamp experiment was conducted on a
Xenopus oocyte to study the conductance o…


QUESTION B:

A patch clamp experiment was conducted on a
Xenopus oocyte to study the conductance of a specific
channel. The membrane voltage was stepped from -90mV to +50 mV and
the following current values were obtained:

Voltage (mV)

Current (pA)

-90

-4

-70

-3

-50

-2

-30

-1

-10

0

+10

+0.25

+30

+0.5

+50

+0.75

1. Plot an I/V relationship using the values in the
above table. Please include all axes labels. [4]

2. At what potential does the current reverse from
inward to outward? What is this called?

3. Calculate the conductance of the channel when the
current is flowing inward.

Show all your calculations

4. Calculate the conductance of the channel when the
current is flowing outward.

Show all your calculations
.

Conductance = I/driving force

5. Is the channel a rectifying or non-rectifying
channel. Explain your answer [2].

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